WISE journal entry #7

Name: Leah Gerald

Entry: #7 (3/6/2012)

WISE Week: 4

Written on Date:3/11/2012

Mentor: Sagar


today was a very short day, partially because we left early, partially because I ended up not doing very much. I was in the lab for an hour less, plus my mentor had a meeting, so I ended up listening a lot. the trays I pipetted last week showed an inconsistency that proved to be problematic. we did 2 sets of each dilution, and basically one of the set of dilutions showed two different results, which doesn't make sense, so they had to re-pipette the whole thing. the week before, my mentor asked me to look up trypan blue, and today, I was quizzed on it. trypan blue is basically a dye they use that colors dead cells blue, because the dead cells allow it to pass through their membrane whereas the live cells do not. they weren't using it today, but in the future they will.