WISE journal entry #8

Name: Leah Gerald

Entry: #8 (3/7/2012)

WISE Week: 4

Written on Date:3/11/2012

Mentor: Sagar


today was a day for watching. According to my mentor today we were extracting RNA. first, we had to take the cells, wash them, and then put trizol on them. the trizol would seperate all the parts of the cell. I got to see it in action under the microscope and it was pretty cool. I could see all of the parts of the cell breaking into tiny little pieces. once all that was done, we had to put a little chloroform in each of the viles, and then put it in the centrifuge. after that, we put a little isopropyl in them, and they were to sit in the freezer for a while, so I didnt get to see the final results. its sad when I dont get to see things to the end, and when I miss things. sometimes I wish i could just come in for a second so I could see the results, because I really wanted to see the RNA.all I was able to actually do that day was wash out the containers, because they had to make sure everything was done very precisely. It was a little reminiscent of my first days, because I spent most of the time watching, and following, and not understanding the humor.