Name: Leah Gerald
Entry: #14 (4/10/2012)
WISE Week: 7
Written on Date:4/10/2012
Mentor: Sagartoday, we were using the western blots previously formed to make one of these:
in order to produce these images, we have to go through a photo-like procedure. first, I washed the membranes in a buffer three times to make sure they were clean. then, we mixed in rabbit antibodies and mouse antibodies, one in each of the trays that corresponded with whichever type of cells. we let those sit for a little while, and while we did that, we went upstairs to get some pancreatic cancer cells. my mentors had found out that there is a gene, Brachiary, seperate from the hippo gland, that can also have an affect on YAP proteins. they found this gene was present in Cordoba cells, which they have in the lab, but recently, they found out that it is also present in pancreatic, colon, and some forms of lung cancer. because of this they were hoping to begin to observe this gene in action in the other types of cells, so I cultured the pancreatic cells into its proper media, and stored it with the others. after this, the western blots were ready to be developed. we poured out the antibodies, and washed them in a sort of photo-developing chemical that brings out light where proteins are present when developed. we then went into another lab, where there is a room to develop westerns, placed them in a machine that does all the developing work for you, and make a couple of images to compare the proteins. at this point in time, I was late for pickup, and I had to go.
No comments:
Post a Comment