Name: Leah Gerald
Entry: #15 (4/11/2012)
WISE Week: 7
Written on Date:4/11/2012
Mentor: Sagartoday went by very fast because I was able to do a lot in the hood. we started out by checking on the pancreatic cells I set yesterday. Sagar said they looked very good, and then I got a chance to look at them
they looked very healthy, and somewhat more different from all the other cells they culture, but because they express that common gene (brachiary) they share at least one similarity. after checking on this, they needed to put some new media in a large amount of the Cordoma cells, and they needed to culture some other cells because they had become too confluent. first, I aspirated all the containers and then pip-petted the new media into each container. it was a long process because there were a lot that needed new media, and as I did this, I didn't realize how much time had passed. the process was very menial and boring, but time just flew by. next, there were some cells that were getting cramped in their container, so we aspirated the media, added trypsan so the cells would un-stick from the bottom of the plate, spun down the cells, mixed them with new media, and re-plated them. the process is one I have watched done several times, except this time, I was able to take part in it as well. at this point, it was time to go, so my day was done.
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