Name: Leah Gerald
Entry: #19 (5/1/2012)
WISE Week:10
Written on Date:5/6/2012
Mentor: Sagartoday, I spent the first part of the day researching fixation, immuno-florescence, and H & E staining, and their connection, because earlier that week, my mentor had recieved some mouse brains from germany which he was staining with H & E. basically, first they had to fix the brains, which causes them to not deteriorate. then, once they are fixed, they stain them with H &E, which colors the nuclei of the cells blue and the other parts red. after this, I went into the hood with Nate, who was replated cells once again, but something else exciting was going on that day, so I was between two things. in the other room, some of the other lab members were preparing to inject some mice with some cancer cells, so my mentor told me I could watch if it interested me. I went to and from the mouse surgery, because It freaked me out a little bit. they pinned the mouse down, cut their skin open, counted down a couple of ribs to the point in the mouses body where its heartbeat was the greatest and injected the cells. at one point, we could see the mouses beating heart under the muscles. I went back to the hood several times because I couldn't watch the whole process. when I did, there were some issues because Nate was replating these cells that showed an irregularity I couldn't quite note.
No comments:
Post a Comment