Name: Leah Gerald
Entry: #20 (5/2/2012)
WISE Week:10
Written on Date:5/6/2012
Mentor: Sagartoday, I had the hood to myself for the first time ever! Sagar had set aside a time and some lung cancer cells that had become too confluent for me to play with that day. I cleaned the station, got out what I would need for my lab, and got to work. I was to take the cells out, count them, dilute them properly, and replate them in 6 different dilutions. first, to get the cells out, I washed them with PBSO, which helps remove calcium, which slows down the trypsin. then I added the trypsin and waited 5 minutes for the trypsin to disconnect all the cells from the bottom of the plate. then I added some media to the mixture to help calm the trypsin down, and spun the cells down in the centrifuge. I then removed the trypsin and media, and mixed the cells with some new media. then I took a small section of this new mixture and counted the cells in the cell counter. I found out how many cells were in one mL, and then had to create 6 different dilutions so that I would have 1 million, 500,000, 50,000, 10,000, 1,000, and 100 cells in each separate well. this part proved very difficult for me, because the mental math was kind of rough for me, and also I never did very well with dilutions in math. so I messed that part up several times and had to redo my dilutions more than once. in the end, I'm not sure I even got it right, but the fact that I did it was enough I guess
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